In a mouse model, researchers found that different probiotic strains can either accelerate or delay the gut microbiome’s recovery after antibiotic treatment.
The work adds to the body of evidence suggesting that probiotic efficacy is specific and situational, and that more research is needed to understand the strain-specific impacts of different probiotics.
Probiotic supplements are often consumed after antibiotic treatment to prevent antibiotic-associated diarrhoeal disease, most commonly caused by Clostridioides difficile (C. diff).
However, the actual impact of individual probiotic strains on the gut’s microbiota, or bacterial environment, is poorly understood.
“Colonisation resistance or the ability to prevent colonisation of pathogens is a function of a healthy microbiota,” says Casey Theriot, Professor of Infectious Disease at NC State and corresponding author of the research.
“This study looked at how long it took resistance against C. diff colonisation to return after antibiotics and the impact of two of the most commonly used commercial strains of Lactobacillus probiotic on that return.”
The research team looked at three groups of mice that had been treated with cefoperazone, a commonly used broad-spectrum cephalosporin antibiotic.
The first group received no probiotic treatment. The second group received L. acidophilus and the third received L. gasseri.
Each group was challenged with C. diff weekly for 4 weeks and the microbiota of each group was examined to measure bacterial load and C. diff resistance.
Mice that received no probiotic showed decreased bacterial load and resistance to C. diff infection by 4 weeks after stopping antibiotics.
However, the L. acidophilus mice had an increased bacterial load in weeks two and three, while the L. gasseri group had no detectable C.diff after 2 weeks.
Additionally, the researchers found that L. gasseri did not colonise or remain in the gut. Instead, it was involved in the production of bacteriocins, or antimicrobial peptides, and it encouraged the growth of Muribaculaceae, another potentially beneficial bacteria.
“We have always known that it’s important to understand the strain-specific impact of probiotic strains,” says Rodolphe Barrangou, Todd R. Klaenhammer Distinguished Professor of Food, Bioprocessing and Nutrition Sciences at NC State and corresponding author of the research.
“Depending on the condition and composition of the individual’s microbiome, the disease, and the probiotic strain, you will have different effects and outcomes.
“What’s interesting is that this study indicates it’s more complicated than people think, because probiotics can have transient or indirect effects on the microbiome."
"L. gasseri doesn’t prevent infection, it transiently promotes recovery of microbiome through Muribaculaceae, which subsequently could provide resistance. This opens new avenues to inform what we should do next.”
“This is the only study out there that is functionally testing resistance in the microbiome,” Theriot adds.
“Although this work is in a mouse model, it shows the need for better mechanistic understanding of how probiotics affect the microbiome, because not only can they have effects weeks after they’ve left the body, in certain situations, they have the potential to prolong or complicate recovery.”