NutriLeads announces a new peer-reviewed in vitro study, conducted in collaboration with dsm-firmenich, demonstrating that its proprietary ingredient Benicaros (carrot rhamnogalacturonan-I or cRG-I) can potentially deliver consistent gut microbiome benefits in dogs.
Published in Microorganisms, the study found that Benicaros significantly increased levels of beneficial short-chain fatty acids (SCFAs) and selectively stimulated Phocaeicola vulgatus — a keystone SCFA-producing species found in the canine gut.
Notably, Benicaros delivered consistent effects across a diverse population of dogs, regardless of size, breed or baseline microbiota composition.
“This consistent production of SCFAs and the ability to balance the gut microbiota composition suggest potential health benefits with Benicaros supplementation in dogs of all sizes,” said Sue McKay, PhD, Nutrition & Health Manager at NutriLeads.
“These findings highlight Benicaros’ value as a next-generation precision prebiotic for companion animal health.”
Study design and key findings
The in vitro study evaluated changes in gut microbiota composition and metabolic activity from 18 healthy dogs representing multiple breeds with small (5–10 kg), medium (10–27 kg) and large (27–45 kg) size categories.
Each faecal sample was treated with Benicaros and compared to treatment with inulin (a commonly used prebiotic with a simple molecular structure and low selectivity) or xanthan (a high-selectivity fibre).
Benicaros stood out for its ability to:
- Consistently stimulate microbial activity and SCFA production — particularly acetate and propionate — across small, mediu, and large dog breeds. Notably, these effects were observed with a low dose and mirror earlier findings in humans.
- Selectively and consistently increase levels of P. vulgatus, a keystone gut microbe, associated with benefits related to gut barrier integrity and immune function in all dog sizes, also mirroring earlier findings in humans.
- Reduce interindividual differences in microbiome response, enabling more consistent and predictable health benefits across diverse dog populations.
In contrast, inulin showed more variable responses across donors, while xanthan had limited fermentability and fewer measurable effects.
“As a partner in this research, we were impressed by the robust and consistent effects of Benicaros on the canine gut microbiota of a broad target population,” said Jeff Alix, Global Head of Business Development, Palatability & Microbiome at dsm-firmenich.
“The ability of cRG-I to selectively enrich beneficial species while reducing variability across different dog sizes is promising."
"These findings not only reinforce the importance of precision prebiotics in dsm-firmenich’s ambition to develop next-generation pet nutrition solutions but also exemplify our commitment to sustainability."
Benicaros is upcycled from carrot pomace — a valuable byproduct of vegetable processing — which directly supports our strategic objective of revalorising production side streams into high-impact, science-backed ingredients.
“Benicaros will allow pet nutrition developers to formulate with confidence, knowing they’re delivering consistent, science-backed benefits,” said Joana Carneiro-Wakefield, PhD, Chief Executive Officer of NutriLeads.